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«ANNUAL REPORT Riga 2012 Annual Report 2011, Institute of Solid State Physics, University of Latvia. Editor: A.Krumins. Composed matter: A.Muratova. ...»

-- [ Page 23 ] --
M.Polakovs,1 N. Mironova-Ulmane,1 A. Pavlenko, 1 E.Reinholds2, M. Grube,3 M.Gavare 3 Institute of Solid State Physics, University of Latvia, Riga, Latvia Nuclear Medicine Dep. P.Stradins Clinical University Hospital, Riga, Latvia Institute of Microbiology and Biotechnology, University of Latvia, Riga, Latvia In the present work we report results of investigations of blood after irradiation by electron paramagnetic resonance (EPR) and infrared spectroscopies. The blood of Chernobyl clean-up workers was investigated using EPR tehnique and blood of patients after radioisotope Tc99m diagnosis was investigated by EPR and FT-IR spectroscopy.

FT-IR absorption spectra of initial (control) and irradiated blood samples were recorded on a micro plate reader HTS-XT (Bruker) in 400-4000 cm-1 region. Data were processed by OPUS 6.5 using hierarchal cluster analysis (HCA), 2nd derivatives, and integration for sample discrimination and quantitative analysis.

The EPR spectra of blood were measured on a BRUKER EMX-6/1 spectrometer equipped with an Aspect 2000 data system. An ER 4102 ST Universal X-Band Resonator (TE102 mode) was used. The g-factors of EPR signals were determined by reference to the external magnetic field value measured by a Bruker ER 035 Gaussmeter and of the microwave frequency measured by a Systron Donner 6235A frequency counter.

HCA discriminated initial and irradiated samples confirming the radiation caused changes in blood. Main changes were detected in intensities and profiles of both amid bands.

Irradiation caused the decrease of protein concentration. Evaluation of initial and irradiated sample IR spectra profiles clearly showed changes in proteins. The ratio of Amid I and Amid II band intensities was significantly higher in initial samples. FT-IR spectra analysis showed that blood proteins are influenced by radiation.

Compared the EPR spectra of human blood not participated in Chernobyl clean-up action and spectra of chernobyl clean-up workers we are confirmed in opinion that chernobyl clean-up workers meet methemoglobin (Fe3+ in high spin state) higher than normal people.

The heightened level of transferrin was registered as well. The EPR spectra of people blood after radioisotope diagnosis show the Fe3+ (in high and low spin states ) higher level compared to spectra of people before radioisotope diagnosis.

EPR and FT-IR spectroscopy could to be powerfull instrument for investigation of influance of radiation on human blood. EPR can detect the concentration of methaemoglobin and transferrin ions of more accurately than any other technique.

EXCITATION WAVELENGTH-DEPENDENT CHANGES IN RAMAN MICROSPECTROSCOPY OF HEMOGLOBIN

–  –  –

In the present work we report the application of the Raman technique to study hemoglobin (Hb) in single erythrocyte using three different laser excitation lines: ultraviolet (441.6), visible (514.5 nm, 532 nm) and near-infrared ( 830 nm). The Raman spectra of the hemoglobin in single erythrocytes were recorded on ”Nanofinder-S” using the 441.6 nm and 532 nm excitation line and on a Renishaw Via instrument using 514.5 nm excitation radiation from a argon laser system and 830 nm from diode laser. Raman excitation line used in 441.6 nm cause resonance enhancement with absorption band 415 nm (Soret band). The local coordinate of this band (pyrrole half ring stretching vibration) involves principally C–N stretching. We report the unusual enhancement of several bands in Raman scattering spectra of hemoglobin at excitation line 830 nm compared with the 441.6, 514.5 and 532 nm wavelengths investigated. The origin of unusual enhancement of Raman scattering bands of hemoglobin will be discussed.

Resonance Raman (RR) spectroscopy is a particularly sensitive probe for studying the electronic and structural properties of metalloporphyrin complexes including haemoglobin (Hb) and myoglobin. The interpretation of the intense spectra obtained from metalloporphyrin complexes has been based on vibronically induced scattering from the B (Soret) or Q states from the porphyrin macrocycle. Resonance Raman scattering from Hb occurs only at its surrounding ligands group because only this part of the molecule absorbs in the visible and near ultraviolet region. Thus it is possible to investigate exclusively vibrations of the four heme groups of Hb without interference by scattering of the surrounding globin or other parts of the red blood cell (RBC) or erythrocyte. In this technique, laser excitation within an electronic absorption band produces selective enhancement of Raman bands associated with vibrations of the protein. In the case of heme proteins, vibrations of the porphyrin ring are enhanced due to resonance with the π-π* transitions which dominate near ultraviolet absorption spectra. (the Soret band) and the visible (the α-β band). Different Raman scattering bands are brought out by excitation in the regions of the Soret absorption band or of the α-β absorption band.

NUCLEAR LEVELS OF 183W STUDIED WITH (n,γ ) AND (d, p) REACTIONS V. Bondarenko d,, I. Tomandl a,b, J. Honzátko b, H.-F. Wirthc,e, T. von Egidy c a Research Centrum, CZ-25068 Rˇež, Czech Republic b Nuclear Physics Institute, CZ-25068 Rˇež, Czech Republic c Physik-Department, Technische Universität München, D-85748 Garching, Germany d Institute of Solid State Physics, University of Latvia, Riga, LV-1063, Latvia e Sektion Physik, Ludwig-Maximilians-Universität München, D-85748 Garching, Germany The level structure of 183W has been studied using gamma–gamma coincidences from thermal neutron capture in 182W accompanied with the reaction (_d, p). From these data and those of previous studies a total of 76 levels and about 490 connecting γ -transitions have been established for energies below 2.4 MeV. An analysis based on the standard distorted-wave Born approximation (DWBA) provides the lj -angular momentum transfers and spectroscopic factors for 36 levels up to 2.2 MeV excitation energy. A large numbe rof particle transitions indicate an influence of strong mixing between particle and probably hole states. The extra exchange of phonons across the Fermi surface leads to a fine structure in the fragmentation of most single particle strengths and at the same time has the effect of breakdown of the individual properties of Nilsson states. The extracted l = 1 and 2 (d, p) sum below 2 MeV has about the same magnitude as in 185,187W. It comprises a rather small fraction of the expected strengths. The observed states below 2 MeV are compared with predictions of the quasiparticle–phonon model. Of particular interest is the identification of “quasi-bands” with inverse spin sequence based on the state 9/2+ at 622.8 keV; this indicates some transition aspects from rigid rotors of light W to γ -soft nuclei in the Os-Pt region.





Scientific Publications

1. V. Bondarenko d, I. Tomandl a,b, J. Honzátko b, H.-F.Wirth,e, T. von Egidy, „Nuclear levels of 183W studied with (n,γ ) and (d, p) reactions” Nucl.Phys. A 856 (2011) p. 1-45.

2. Nina Mironova-Ulmane, Alexei Kuzmin, J. Grabis, I. Sildos, V.I. Voronin, I.F. Bergerand and V.A. Kazantsev. Structural and Magnetic Properties of Nickel Oxide Nanopowders.

Solid State Phenomena Vols, 168-169, 2011, pp. 341-344.

3. D. Riekstina, O.Veveris. Tritium concentration in drinking and ground water in Latvia, LSC 2010, Advances in liquid scintillation spectrometry, Proc of the 2010 Int, LSC, Paris, France, Edit. P. Cassette, Tucson, Arizona, USA, 2011, pp. 95-98.

4. D. Riekstina, J. Berzins, O.Veveris. Conformity assessment in radioactivity measurement.Pros. of The 4th International Conference on Metrology, Jerusalem, Israel, 23-24 May 2011.

5. V Skvortsova, N. Mironova-Ulmane, D. Riekstina, Structute and phase changes in natural and synthetic magnesium aluminium spinel, Proc. of 8th Int. Scient and Pract. Conf. June 20-22, 2011, Rezekne, pp. 100-106.

6. J. Berzins, D. Riekstina, N. Mironova-Ulmane, in “50 years of Salaspils research reactor” (0n Latvian) Riga, Zinatne. 2011.

7. J Proskurins, “Study of Quantum chaos and phase transitions in nuclear models” Doctoral thesis, Riga, 2011.

8. N. Mironova-Ulmane, A. Kuzmin,, J. Grabis I. Sildos, V.I. Voronin, I.F. Berger and V.A.

Kazantsev Structural and Magnetic Properties of Nickel Oxide Nanopowders. Solid State phenomena (2011) Vols.168-169. pp.341-344

9. N. Mironova-Ulmane, A. Kuzmin, I. Sildos, and M. Pars, Polarisation dependent Raman study of single-crystal nickel oxide, Centr. Eur. J. Phys. 9 (2011) 1096-1099.

10. N. Mironova-Ulmane, M. Polakovs, J. Grabis, V. Serga, L.Kulikova, D. Jakovlevs, I.

Sildos, Mairov. Raman spectroscopy and magnetic properties nanosized of CoFe2O4.

Proc. Int. Conf. "Actual problems of solid state physics-2011", 18-21 October 2011, Minsk, Belorus,.pp.101-103.

11. V. Skvortsova, L. Trinkler. Transition metal ions luminescence in neutron irradiated magnesium oxide. IOP Conference Series: Materials Science and Engineering, 2010, v.

15, p.012055(1-6).

12. V. Skvortsova, N. Mironova-Ulmane, D. Riekstiņa. Structure and phase changes in natural and synthetic magnesium aluminium spinel, “Environment. Technology. Resources”, Proceedings of the 8th International Scientific and Practical Conference, Rēzeknes Augstskola, Rēzekne, RA Izdevniecība, 2011, vol. 2, pp. 100-106.

13. N. Mironova-Ulmane, Radiation-induced processes in oxides Proc. Int. Conf. "Actual problems of solid state physics-2011", 18-21 October 2011, Minsk, Belorus,.pp. 20-22.

Conference Presentations

1. J.Bērziņš, M. Balodis, T.Krasta Nuclear structure studies at Salaspils reactor, LU CFI 27.

sci.. conf.. 2011 g. 14.-16. Febr. Abstracts, p.17.

2. J.Bērziņš, M. Balodis, T.Krasta, V. Bondarenko, Ļ. Simonova Structure studies of A~190 transitional region odd-odd nuclei, LU CFI 27. sci. conf. 2011 g. 14.-16. Febr.

Abstracts,p.18.

14. D.Riekstiņa. Neutron activation analysis in Salaspils nuclear reactor. LU CFI 27. sci..

conf.. 2011 g. 14.-16. Febr. Abstracts, p.19.

15. D.Riekstiņa, J. Berzins, O.Veveris. Conformity assessment in radioactivity measurement. The 4th International Conference on Metrology, Jerusalem, Israel, 23-24 May 2011.

16. M.Balodis, J.Bērziņš, V.Bondarenko, T.Krasta, L.Simonova M.Jentschel, W.Urban Tomandl, „Thermal neutron capture study of 188Re levels”, CGS-14 (The 14th International Symposium on Capture Gamma-Ray Spectroscopy and Related Topics)Guelph, Canada, August 28 – September 2, 2011.

17. D.Riekstiņa, J.Berzins, O.Veveris, Application of gamma spectrometry and LSC in control of environment. Programme quide & book of abstracts 14th International Congress of Radiation Research, Warsaw, Poland, 28.08 - 01.09, 2011, pp. 170-17.

18. N. Mironova-Ulmane Radiation-induced processes in solids Abstracts of the 27th Scientific Conference g. February 14.-16. febr.2011 p.20.

19. V. Skvortsova. Luminescence of chromium and iron ions in magnesium oxide crystals.

Abstracts of the 27th Scientific Conference. February 14.-16. febr.2011 p. 84.

20. G.Dobele, T.Dizhbite, V.Jurkjane, N.Mironova-Ulmane, I.Sildos, T.Centeno. Nanoporous Carbon Materials for Supercapacitor Electrodes. International Baltic Sea Region conference „Functional materials and nanotechnologies FM&NT-2011” 5-8 April, Riga, Latvia, p.235

21. N.Mironova-Ulmane A. Kuzmin I.Sildos. Temperature Dependent Raman Study of NiWO4. International Baltic Sea Region conference „Functional materials and nanotechnologies FM&NT-2011” 5-8 April, Riga, Latvia, p.114

22. T. Dizbite, G. Telysheva, L. Jashina, G. Dobele, A. Volperts, A.Andersone, J.Ponomarenko, N. Mironova-Ulmane, I. Sildos. Lignin Oxidation Using Heteropolyanions of the Keggin’s Type as Catalysts. International Baltic Sea Region conference „Functional materials and nanotechnologies FM&NT-2011” 5-8 April, Riga, Latvia, p. 261.

23. M. Polakovs, N. Mironova-Ulmane, A. Pavlenko, T. Zvagule, N. Kurjane, N.Gabrusheva Study of iron proteins in blood of Chernobyl clean-up workers by EPR.. International Baltic Sea Region conference „Functional materials and nanotechnologies FM&NT-2011” 5-8 April, Riga, Latvia, p. 115.

24. N. Mironova-Ulmane, M. Polakovs, J. Grabis, V. Serga, L.Kulikova, D. Jakovlevs, I.

Sildos.Raman spectroscopy of CoFe2O4.. International Baltic Sea Region conference „Functional materials and nanotechnologies FM&NT-2011” 5-8 April, Riga, Latvia, p.274.

25. M.Polakovs, N. Mironova-Ulmane, A. Pavlenko, E.Reinholds, M. Grube, M.Gavare. EPR and FTIR spectroscopies studyof human blood after irradiation. 14th European Congress on Spectroscopy of Biological molecules (ECSBM-11), Coimbra, Portugal, August 29t August - 3rd September 2011, p.44.

26. M.Polakovs, N. Mironova-Ulmane, I. Sildos excitation wavelength-dependent changes in Raman micro-spectroscopy of hemoglobin 14th European Congress on Spectroscopy of Biological molecules (ECSBM-11), Coimbra, Portugal, August 29t August - 3rd September 2011, p.4.

27. M.N. Palatnikov, A.N. Salak, K. Bormanis, O.B. Scherbina, V.V. Efremov, N.V. Sidorov, I.N. Efremov, and N. Mironova-Ulmane. Structure and Elastic Properties of Sodium Niobate Ceramics Prepared at High Pressure. 16th-semiconducting-and-insulatingmaterials-conference ( SIMC-16) Tu3-31.

28. K. Bormanis, A. Kalvane, A.I. Burkhanov, I.E. Tumanov, and N. Mironova-Ulmane Dielectric properties of PMN+2%Li2O at low and infra-low frequencies. 16thsemiconducting andinsulating-materials-conference ( SIMC-16) Tu3-33.

29. M.N. Palatnikov, K. Bormanis, V.V. Efremov, N.V. Sidorov, I.N. Efremov, and N.

Mironova-Ulmane. Dielectric Properties of Lithium-Sodium Tantalate-Niobate Solid Solutions. 16th-semiconducting-and-insulating-materials-conference ( SIMC-16) Tu3-31.

Physics Doctor students:

1. Jevgenijs Proskurins – Study of quantum chaos and phase transitions in nuclear models.

Supervised by Dr.T.Krasta. the thesis were preentedin June 2011.

.

Lectures at Universities, Institutes …

During 2011, T. Krasta was engaged in following activities related with LU study programs:

1) supervision of J. Proskurins physics doctor dissertation work „Theoretical study of quantum



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